According to the statement from the Souza Baranowski Correctional Center in Shirley Massachusetts, Hernandez hanged himself with a bed sheet that he attached to a cell window.
Hernandez was found dead with the words "John 3:16" written on his forehead, according to a law enforcement source directly involved in the investigation into the former National Football League star's death.
Investigators say Hernandez blocked access to his cell from the inside by jamming cardboard into the door tracks.
"Nields performed an autopsy on Mr. Hernandez on Wednesday and concluded today that the manner of death was suicide and the cause asphyxia by hanging", the Worcester County District Attorney's office said.
The New England Patriots, the Super Bowl LI champions, are scheduled to visit the White House this afternoon to meet with President Donald Trump.
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Hernandez had been expected to appeal his sentence of life in prison without possibility of parole for the 2013 murder. The former NFL-er was found hanged in his jail cell by prison officials and pronounced dead early that morning.
Both the Massachusetts State Police and prison plan to do a formal investigation into Aaron's passing.
John M. Thompson, the attorney handling Hernandez's appeal in the 2013 murder case, also said Hernandez's death should be "carefully and thoroughly investigated".
Massachusetts Gov. Charlie Baker told reporters Thursday that any time someone takes their own life in prison "something clearly went wrong".
Legally speaking, Aaron Hernandez may have become a free man in his death.
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All projects, including Marshall, the area hospitals and the city's downtown, will be connected to high speed internet access. Williams and other city officials were in Denver, Colorado to present the city's revitalization plans to a panel of judges.
Jose Baez, the family's attorney, said arrangements were already made to donate the football player's brain to Boston University's Chronic Traumatic Encephalopathy Center, a world renowned leader in CTE research.
The child molestation conviction of former Roman Catholic priest John Geoghan, a key figure in the clergy sex abuse scandal that rocked the Boston archdiocese, was vacated after he was beaten to death in 2003 in his cell at the same MA maximum-security prison. Hernandez's apparent suicide left friends, family and his legal team shocked and in disbelief.
Last Friday, Hernandez was found not guilty for the double murder of two men on a Boston street in 2012.
He said the brain was supposed to be picked up at 10 a.m. Thursday, but the medical examiner's office reversed course at the last minute and chose to keep the brain and conduct its own study.
Baez said his team did not know for certain if Hernandez suffered from CTE or if it contributed to his death. Some of Hernandez's representatives believe his death wasn't a suicide.
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The mother of Odin Lloyd, who was murdered in 2013, will move forward with her lawsuit against Hernandez, according to TMZ Sports. Hernandez was born in Bristol, Connecticut, and was a star tight end at the University of Florida.